Year : 2017  |  Volume : 25  |  Issue : 2  |  Page : 86-89

The effect of povidone–iodine on normal bacteria conjunctival flora in adult patients in Onitsha Nigeria

1 Guinness Eye Centre Onitsha, Onitsha, Nigeria
2 Guinness Eye Centre Onitsha, Onitsha; Department of Ophthalmology, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria
3 Department of Medical Microbiology, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria

Correspondence Address:
Sebastian N.N Nwosu
Guinness Eye Centre Onitsha, PMB 1534, Onitsha
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/njo.njo_38_16

Rights and Permissions

Objectives: To determine the normal conjunctival flora and the effect of povidone–iodine on the flora in adult patients at the Guinness Eye Centre Onitsha Nigeria. Materials and Methods: Each randomly recruited new adult patient had conjunctival swab taken from one eye; then a drop of 5% povidone–iodine solution was instilled on the conjunctiva. Conjunctival swabs were taken at 1, 5, and 10 min after povidone–iodine instillation. The swabs were smeared on glass slides and also inoculated unto MacConkey, chocolate, and nutrient agar. Microbial study of the specimens was undertaken. Results: Of the 250 eyes of the 250 patients, 164 (65.6%) were culture-positive. A total of 225 organisms were isolated from the 164 culture-positive specimens as follows: Pseudomonas aeruginosa was isolated in 142 (63.1%), Staphylococcus epidermidis in 79 (35.1%), and Staphylococcus aureus in four (1.8%). Among the 61 specimens that grew more than one organism, 59 (96.7%) had a mixed growth of P. aeruginosa and S. epidermidis and 2 (3.3%) grew both S. epidermidis and S. aureus. Povidone–iodine significantly reduced the mean bacterial colony count in all bacterial isolates 10 min after instillation, that is: 114.4 to 35.7 for P. aeruginosa (P < 0.05), 71.7 to 15.8 for S. epidermidis (P < 0.05), and 45.5 to 3.0 for S. aureus (P < 0.05). Bacterial colonies persisted in seven (2.8%) eyes 10 min after instillation of 5% povidone–iodine. Conclusion: The normal conjunctiva of some patients harbor virulent bacteria, which load 5% povidone–iodine greatly reduced. These facts should be taken into consideration when planning intraocular surgery as part of the efforts to prevent endophthalmitis.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded267    
    Comments [Add]    

Recommend this journal